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[\/vc_message][om_agenda][om_agenda_day color=”om-accent-color-3″ title=”QUINTA-FEIRA – SALA A – S\u00cdNCRONA” date=”21\/10\/2021″][om_agenda_item speaker_links=”” time=”11 h – 11 h 20 min” title=”`{`208308`}` Ensuring the validity of measurement results of nitrite and nitrate in meat products” speaker_ids=”1446″]Autores: Mariana Cardoso de Oliveira, Vanderl\u00e9a de Souza, Marcelo Dominguez de Almeida<\/p>\n
Palavras-chave: nitrate, nitrite, meat product, validation of method<\/p>\n
Resumo: Nitrate and nitrite measurement is a fundamental part of quality control in the meat products industries. Ensuring the technical validity of the results of these measurements can be achieved through the adoption of appropriate metrological tools such as method validation. In this study, the measurement of the mass fractions of nitrite and nitrate ions was performed by ion chromatography with UV detection. The method was linear (r > 0.99) over the working range. Limits of quantification values for measuring nitrite and nitrate in meat product were 10 mg kg-1 and 100 mg kg-1 , respectively. The mean values of recovery at all evaluated mass fraction levels were within the range of 88 % to 106 %. All the validation parameters resulted as satisfactory and the method was considered adequate for the intended use.<\/p>\n
Palavras-chave: linearity, limit of detection (LOD), limit of quantification (LOQ), working range, energy dispersive X-ray fluorescence (EDXRF)<\/p>\n Resumo: Using a portable energy dispersive X-ray fluorescence (EDXRF) spectrometer and certified reference materials, the linearity, the limit of detection (LOD), the limit of quantification (LOQ), and the working range of the method were determined for the quantitative analysis of base and precious metals in liquid matrices. As the EDXRF method is not standardized and strongly sensitive to matrix effects, these calculations are essential to its analytical validation. In this study, the resulting R2 values were higher than 0.99 and the residuals had random distribution. The LOD and LOQ values were considered low, close to the resolution of the spectrometer. Thus, our calculations confirmed the analytical validation of the portable EDXRF method in liquid matrices. The utilization of portable EDXRF equipment in the quantitative measurement of elements in liquid matrices is innovative and reveals new fields of application for this type of instrument.<\/p>\n Palavras-chave: Hydrous ethanol fuel, ethanol pH, combined glass electrode, pH indicator<\/p>\n Resumo: The pH is one of the quality parameters of hydrous ethanol fuel (HEF), being established by Brazilian standard ABNT NBR 10891 and by American standard ASTM D 6423. The present work aims to compare the measurement systems proposed by the standards for measuring pH in hydrous ethanol fuel and compare them with the pH range found from pH indicators, considered as a standard measurement. The results reveal that the pH values obtained by the different systems used are statistically different from each other and different from the range found by the indicators.<\/p>\n Palavras-chave: nitrate, meat product, reference material, feasibility study.<\/p>\n Resumo: Nitrate measurement is a fundamental part of quality control in the meat products industries. Ensuring the technical validity of the results of these measurements can be achieved through the adoption of appropriate metrological tools such as reference materials. The technical feasibility for producing a nitrate reference material in a meat product was evaluated by preparing a batch of material on a small scale. The adequacy of the preparation procedure was evaluated by means of pertinent physicochemical and microbiological measurements. A batch of 175 units containing approximately 5 g of freeze-dried ham in each package was produced. The batch was submitted to preliminary studies to assess homogeneity and stability under transport conditions, in accordance with ISO 17034 and the applicable ISO guides.<\/p>\n Palavras-chave: peixe, ensaios de profici\u00eancia, qualidade.<\/p>\n Resumo: Para que o crescimento e desenvolvimento da cadeia pesqueira aconte\u00e7a de maneira sustent\u00e1vel, \u00e9 necess\u00e1rio o desenvolvimento de bases cient\u00edficas e tecnol\u00f3gicas s\u00f3lidas, que auxiliem na garantia da qualidade desta mat\u00e9ria prima. Uma das principais maneiras de comprovar a qualidade destes produtos \u00e9 fornecida por meio de resultados anal\u00edticos. Neste contexto, o desenvolvimento de materiais de refer\u00eancia destinados a ensaios de profici\u00eancia em matriz de pescado contribui para o avan\u00e7o cient\u00edfico, tecnol\u00f3gico e de qualidade desta mat\u00e9ria prima. Este trabalho descreve as etapas de produ\u00e7\u00e3o de um material de refer\u00eancia destinado a ensaios de profici\u00eancia em matriz pescado. O estudo abrange as etapas de sele\u00e7\u00e3o do material, prepara\u00e7\u00e3o e desenvolvimento dos estudos de homogeneidade e estabilidade a curto prazo simulando condi\u00e7\u00f5es de transporte. A avalia\u00e7\u00e3o dos resultados foi apresentada por meio de desvio padr\u00e3o de Horwitz e desvio padr\u00e3o pela experi\u00eancia de rodadas anteriores de programas de ensaio de profici\u00eancia, realizando-se compara\u00e7\u00f5es entre ambos. Verificou-se que o lote de material produzido se apresentou homog\u00eaneo e se manteve est\u00e1vel durante o estudo de estabilidade, possibilitando sua utiliza\u00e7\u00e3o em ensaios de profici\u00eancia.<\/p>\n Palavras-chave: Validation, Cell count, Neubauer chamber, Automatic count.<\/p>\n Resumo: The quantification of biological processes has gained importance in metrology, as its application in diagnosis and personalized therapy has been increasing the demand of the biotechnology sector. Considering that the cell is the microscopic and functional unit of living beings, cell count is critical for the reproducibility of the in vitro study. This work aims to determine the measurement uncertainty and the commutability of the methods of manual cell counting in a Neubauer chamber and automatic cell counting, both image-based and widely used. For this, we used different amounts (1×105 -6×105 ) of HCT-116 cells, a well-established line whose authenticity was confirmed by STR analysis. Initially, we checked if 20 up\/down movements, performed with the micropipette, are enough to maintain the homogeneous distribution of cells in the four most outer quadrants of the Neubauer chamber. Then, different amounts of cells (1×105 -6×105 ) were prepared using a balance or calibrated micropipettes, stained with trypan blue (1:1) and counted in an automatic counter or a Neubauer chamber, in quadruplicate. Data analysis was performed using the GraphPad Prism 6 program, and the significance analysis was performed using the one-way ANOVA test, Bonferroni post-test, and simple linear regression. The measurement uncertainty was estimated using the calculations recommended in the ISO GUM. The results show that the homogenization provided an equitable distribution (p>0.05) of cells in the four most outer quadrants of the Neubauer chamber, regardless of the cell concentration used. Manual and automatic counting showed the same linear correlation between the expected cell number and the observed cell number, showing the compatibility of the methods. As a function of the cell concentration analyzed, the measurement uncertainty ranged between 6-18% for automatic counting and between 11-24% for manual counting. However, we observed that the commutativity of the manual and automatic counting methods depends on the dilution preparation method and that only the gravimetric dilution presented commutativity (p>0.05). The results show that performing 20 up\/down movements are sufficient to homogenize the samples for counting in quadruplicate in the Neubauer chamber and the automatic counter, that counting in 4 and 9 quadrants are equivalent, that the manual and automatic methods are switchable when the dilutions are prepared by gravimetry and have similar measurement uncertainty, in the range between 6 and 24%. We intend to use these results to validate manual counting methodology and automatic counting of human cells, allowing a possible comparison with other automated methodologies, including flow cytometry and realtime electrical impedance analysis.<\/p>\n Palavras-chave: Validation; ddPCR; cancer<\/p>\n Palavras-chave: Nanotoxicidade, Nanopart\u00edcula de prata, Hep-G2, Viabilidade celular.<\/p>\n Resumo: O crescimento exponencial da nanotecnologia na pesquisa e no mercado \u00e9 algo not\u00e1vel, sendo as nanopart\u00edculas de prata (AgNPs) uma das mais exploradas. J\u00e1 \u00e9 conhecido que AgNPs possuem efeitos citopatot\u00f3xicos frente \u00e0s c\u00e9lulas de f\u00edgado humano (HEP-G2). Baseado nisso, esse estudo buscou avaliar a altera\u00e7\u00e3o da viabilidade celular (HEP-G2) frente \u00e0 exposi\u00e7\u00e3o de diferentes concentra\u00e7\u00f5es de AgNPs (0,001 – 10 \u00b5g\/mL) no per\u00edodo de 48h de exposi\u00e7\u00e3o utilizando testes de viabilidade (MTT e LDH). Inicialmente, as AgNPs foram caracterizadas pelos m\u00e9todos de espalhamento de luz din\u00e2mico (DLS) e microscopia eletr\u00f4nica de transmiss\u00e3o (MET). Os resultados demonstraram que as nanopart\u00edculas utilizadas apresentam morfologia esf\u00e9rica com di\u00e2metro m\u00e9dio de 20\u00b110 nm. Ap\u00f3s a intera\u00e7\u00e3o celular foi poss\u00edvel observar por MTT redu\u00e7\u00e3o de viabilidade nas concentra\u00e7\u00f5es de 1; 2,5; 5; 7,5 e 10 \u00b5g\/mL, alcan\u00e7ando uma redu\u00e7\u00e3o pr\u00f3xima dos 50% de viabilidade com IC50>10 \u00b5g\/mL. O ensaio de LDH destacou que n\u00e3o h\u00e1 altera\u00e7\u00e3o na libera\u00e7\u00e3o de lactato desidrogenase para o meio extracelular. Esses resultados revelam que mesmo nas menores doses houve altera\u00e7\u00e3o considerada significativa da viabilidade e, que possivelmente, as c\u00e9lulas n\u00e3o sofrem lise de membrana. Esses dados compilados ir\u00e3o contribuir para informa\u00e7\u00f5es nanot\u00f3xicol\u00f3gicas e, consequentemente, de forma indireta para o processo de padroniza\u00e7\u00e3o das t\u00e9cnicas utilizadas.<\/p>\n Palavras-chave: Valida\u00e7\u00e3o de m\u00e9todo, RNA, ddPCR, BVDV, flavivirus<\/p>\n Resumo: A increasingly common way for the diagnostics of viral agents is to carry out PCR from biological samples, which can detect their nucleic acid during the acute phase of the disease. Taking into account the need for quality control of PCR tests in laboratories accredited by ISO\/IEC 17025:2017, we intend to carry out feasibility studies for the production of Reference Materials (MR) for these molecular tests, using BVDV in the matrix serum as a model. The first step for this goal was the validation of an analytical method for quantification of viral RNA to characterize the material under study. Primers and probe from a commercial qualitative assay kit designed for Real Time RT-PCR (VetMax Gold BVDV – Thermo Fisher) were used for quantification of BVDV by ddPCR. The technique was optimized and a Detection Limit of 13 copies\/\u00b5L was determined, allowing a Quantification Limit of 38 copies\/\u00b5L. The method showed to be linear over two orders of magnitude and will be used in the homogeneity study and long-term stability tests for the pilot batch of BVDV in Fetal Bovine Serum MR, as a prototype for the production of MR intended for PCR of BVDV and other Flaviviridae.<\/p>\n Palavras-chave: volumetria, incerteza de medi\u00e7\u00e3o, ensaios biol\u00f3gicos, in vitro, micropipetas<\/p>\n Resumo: A incerteza na determina\u00e7\u00e3o volum\u00e9trica de instrumentos como as pipetas, entre outros, \u00e9 um dos dados mais relevantes para a garantia da qualidade de ensaios biol\u00f3gicos com c\u00e9lulas, podendo ser impactada por par\u00e2metros sistem\u00e1ticos e aleat\u00f3rios. Foram comparadas estrat\u00e9gias de remo\u00e7\u00e3o de solu\u00e7\u00f5es das placas de cultura de c\u00e9lulas comumente utilizadas, incluindo ou n\u00e3o o uso de micropipetas, para avaliar a repetibilidade de tais m\u00e9todos e quantificar a incerteza de medi\u00e7\u00e3o associada. Removendo solu\u00e7\u00f5es por invers\u00e3o, n\u00e3o vemos diferen\u00e7a significativa nos volumes obtidos entre as rodadas, dentro das mesmas condi\u00e7\u00f5es. As micropipetas multicanal apresentaram valores de incerteza expandida diferentes entre os canais. A escolha da estrat\u00e9gia de enx\u00e1gue de solu\u00e7\u00f5es em seus protocolos, portanto, impacta no aumento da incerteza associada ao c\u00e1lculo do resultado de medi\u00e7\u00e3o em ensaios biol\u00f3gicos com c\u00e9lulas.<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>[\/om_agenda_item][om_agenda_item speaker_links=”” time=”11 h 20 min – 11 h 40 min” title=”`{`204973`}` Portable EDXRF spectrometer: determination of linearity, LOD, LOQ, and working range for the analysis of base and precious metals in liquid matrices” speaker_ids=”1446″]Autores: Alex Krummenauer, Hugo Marcelo Veit, Marcelo Pilotto Cenci, Luis Henrique Lapazin<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>[\/om_agenda_item][om_agenda_item speaker_links=”” time=”11 h 40 min – 12 h” title=”`{`204911`}` pH measurements fuel hydrated ethanol” speaker_ids=”1446″]Autores: Natalia Cambiaghi Atilio, Elcio Cruz de Oliveira, Fernando Luis Fertonani<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>[\/om_agenda_item][om_agenda_item speaker_links=”” time=”12 h – 12 h 20 min” title=”`{`205069`}` Technical Feasibility Study for Development of Reference Material for Nitrate in Meat Product” speaker_ids=”1446″]Autores: Mariana Cardoso de Oliveira, Vanderl\u00e9a de Souza, Marcelo Dominguez de Almeida<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>[\/om_agenda_item][om_agenda_item speaker_links=”” time=”12 h 20 min – 12 h 40 min” title=”`{`206778`}` Desenvolvimento de material de refer\u00eancia em matriz pescado destinado a ensaio de profici\u00eancia f\u00edsico-qu\u00edmico” speaker_ids=”1446″]Autores: Marina Zuffo, DAIANE CHIARELLO, JULYANA PITTOL MARTINS, JOSEANE CRISTINA BASSANI, MAICON RODRIGO Zangalli<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>[\/om_agenda_item][om_agenda_item speaker_links=”” time=”14 h – 14 h 20 min” title=”`{`208176`}` Validation of human cell counting methods” speaker_ids=”1451″]Autores: Daniella Paiva Bonfim, Bruno Cosme, Jailton Carreteiro Damasceno, Jos\u00e9 Mauro Granjeiro, Fernanda Leve<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>[\/om_agenda_item][om_agenda_item speaker_links=”” time=”14 h 20 min – 14 h 40 min” title=”`{`206883`}` Validation of digital PCR for detection of mutations in colon and lung cancer” speaker_ids=”1451″]Autores: Giulia Gall delgado de souza, Bruno Cosme, Jos\u00e9 Mauro Granjeiro, Fernanda Leve<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>[\/om_agenda_item][om_agenda_item speaker_links=”” time=”14 h 40 min – 15 h” title=”`{`204969`}` Avalia\u00e7\u00e3o da viabilidade de c\u00e9lulas hep\u00e1ticas (HepG2) ap\u00f3s tratamento com nanopart\u00edculas de prata” speaker_ids=”1451″]Autores – B\u00e1rbara Gomes de Carvalho, Wanderson de Souza, Lorena Gon\u00e7alves Henriques Corr\u00eaa Maduro, Camila Brand de Carvalho, Celso Barbosa de Sant\u2019Anna Filho, Jos\u00e9 Mauro Granjeiro, Leonardo da Cunha Boldrini Pereira, Priscila Grion<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>[\/om_agenda_item][om_agenda_item speaker_links=”” time=”15 h – 15 h 20 min” title=”`{`208866`}` Validation of a Bovine Viral Diarrhea Virus (BVDV) absolute quantification method by digital droplet PCR (ddPCR)” speaker_ids=”1451″]Autores: Mayane Ribeiro de Faria Henrique, Aurea Valadares Folgueras Flatchart, Vanderlea de Souza, Jose Mauro Granjeiro, Roberto Becht Flatschart, Flavio Guimar\u00e3es da Fonseca, Jos\u00e9 Lafaiete Palles Ramos J\u00fanior, Shana Priscila Coutinho Barroso, Marcos Bryan Heinemann<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>[\/om_agenda_item][om_agenda_item speaker_links=”” time=”15 h 20 min – 15 h 40 min” title=”`{`205516`}` Avalia\u00e7\u00e3o da repetibilidade e incerteza associada aos m\u00e9todos de enx\u00e1gue de c\u00e9lulas em ensaios biol\u00f3gicos in vitro” speaker_ids=”1451″]Autores: Lorena de Oliveira Neves, Bruno Carius Garrido, Jose Mauro Granjeiro, Luciene Bottentuit L\u00f3pez Balottin<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>[\/om_agenda_item][\/om_agenda_day][\/om_agenda][om_agenda display_room=”” display_speakers=””][om_agenda_day color=”om-accent-color-2″ title=”Coordenadoras: Vanderl\u00e9a de Souza e \u00c1urea Valadares” el_class=”om-agenda-day-header-assicrona”][\/om_agenda_day][om_agenda_day color=”om-accent-color-2″ title=”ASS\u00cdNCRONA”][om_agenda_item icon_fontawesome=”fa fa-check” speaker_links=”” title=”`{`205072`}` Estudo do m\u00e9todo de liofiliza\u00e7\u00e3o como alternativa de conserva\u00e7\u00e3o de leite flu\u00eddo no desenvolvimento de material de refer\u00eancia para ensaio de profici\u00eancia f\u00edsico-qu\u00edmico”]Autores: Marina Zuffo, Maicon Rodrigo Zangalli, Joseane Cristina Bassani<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>
\n<\/span>[\/om_agenda_item][om_agenda_item icon_fontawesome=”fa fa-check” speaker_links=”” title=”`{`209951`}` Avalia\u00e7\u00e3o da citotoxicidade de nanopart\u00edculas usadas nas ind\u00fastrias biotecnol\u00f3gicas”]Autores: Priscila G.M. Borchio, Wanderson de Souza, Leonardo Boldrini, Ana R. Ribeiro, Jos\u00e9 Mauro Granjeiro<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>
\n<\/span>[\/om_agenda_item][om_agenda_item icon_fontawesome=”fa fa-check” speaker_links=”” title=”`{`209925`}` Detection of Microbiological Contamination in Cell Cultures and their Raw Materials: a 6 years report of this activity”]Autores: Jos\u00e9 Lafaiete Palles Ramos Junior, Oscar de Souza Monteiro, Nathalia Correia dos Santos, Raquel Correia, Danielle P Cavalcanti, Juliana L Martins, Leonardo da Cunha Boldrini Pereira, Jos\u00e9 Mauro Granjeiro, Aurea Valadares Folgueras-Flatschart<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>
\n<\/span>[\/om_agenda_item][om_agenda_item icon_fontawesome=”fa fa-check” speaker_links=”” title=”`{`209841`}` An\u00e1lise preliminar do modelo de pele equivalente para teste de irrita\u00e7\u00e3o cut\u00e2nea”]Autores: Priscila Laviola Sanches, Luths R O Geaquinto, Marcio Lorencini, Desiree C Schuck, Bruna B S Sievers, Meg C C Costa, Carolina M Catarino, Ana R L Ribeiro, Jose M Granjeiro<\/p>\n
\n<\/i> P\u00e1gina do Artigo<\/span><\/a>
\n<\/span>[\/om_agenda_item][om_agenda_item icon_fontawesome=”fa fa-check” speaker_links=”” title=”`{`208246`}` Caracteriza\u00e7\u00e3o de nanopart\u00edculas de s\u00edlica amorfa para ensaios de citotoxicidade”]Autores: Beatriz Kopke de Assis Dal-Cheri, Wanderson de Souza, Celso Barbosa de Sant’Anna Filho, Lu\u00eds Mauricio Trambaioli da Rocha e Lima, Jos\u00e9 Mauro Granjeiro, Paulo Em\u00edlio Corr\u00eaa Leite, Leonardo da Cunha Boldrini Pereira<\/p>\n