{"id":2237,"date":"2021-12-08T13:14:27","date_gmt":"2021-12-08T16:14:27","guid":{"rendered":"https:\/\/metrologia2021.org.br\/?p=2237"},"modified":"2021-12-08T13:14:27","modified_gmt":"2021-12-08T16:14:27","slug":"206883-validation-of-digital-pcr-for-detection-of-mutations-in-colon-and-lung-cancer","status":"publish","type":"post","link":"https:\/\/metrologia2021.org.br\/?p=2237","title":{"rendered":"[206883] Validation of digital PCR for detection of mutations in colon and lung cancer"},"content":{"rendered":"

[vc_row][vc_column][vc_text_separator title=”ARTIGO” i_icon_fontawesome=”fa fa-file-pdf-o” color=”om-accent-color-3″ add_icon=”true”][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Autores: <\/strong>Giulia Gall delgado de souza, Bruno Cosme, Jos\u00e9 Mauro Granjeiro, Fernanda Leve<\/p>\n

[\/vc_column_text][vc_separator][vc_column_text]Resumo:<\/strong> The ddPCR is an absolute DNA quantification technology, allowing the identification of rare mutations, however its application in oncology diagnosis requires evaluation of performance parameters. We performed a qualitative validation of ddPCR, producing and using an in-house standard of DNA containing mutations in EGFR (p.L858R) and KRAS (p.G13D), highly incident in lung and colon cancer, respectively. We analyzed the limit of blank (LOB, < 2 copies\/uL), limit of detection (LOD, 10 copies\/uL), selectivity and robustness. Our results show that the ddPCR is a robust and specific method, however the commutativity study is still needed.\n[\/vc_column_text]\n\n
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